Natural Product Chemistry, Pharmacognosy, Alternative Medicine

Laboratory Skills

1. Phytochemistry Extraction, purification and characterization of plant secondary metabolites using different chromatographic and spectrophotometric techniques such as thin layer chromatography (TLC), preparative thin layer chromatography (PTLC), column chromatography (CC), gas chromatography-mass spectrometry (GCMS), liquid chromatography-mass spectrophotometric (LCMS), high performance liquid chromatography (HPLC) and UV spectrophotometer.
2. Biochemistry Biochemical markers such as total carbohydrates, total protein, pepsin content from gastric juice. Hematological parameters such as RBC, WBC, platelet and Hb from blood. Moreover, histopathological assay of different organs.
3. Phytopharmacology In vitro and in vivo quantitative analysis of total antioxidant and ferrous reducing antioxidant capacity, DPPH (1, 1-diphenyl-2-picrylhydrazyl) and hydroxyl radical scavenging assay, lipid peroxidation inhibition assay, iron chelating assay, acid phosphatase (ACP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), total bilirubin, as well as serum total protein, acetylcholinesterase and butyrylcholinesterase and antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GSH) using the standard spectrophotometric and bio analyzer methods.
4. Cell Biology Different cell culture and maintain, cytotoxicity test on brine shrimp nauplii. Cell growth inhibition in EAC-induced tumor bearing mice. MTT assay on HeLa cell.
5. Molecular Biology RNA and DNA isolation and purification, cDNA synthesis, and RT-PCR. mRNA and DNA detection using agarose gel electrophoresis.
6. Molecular Docking Computer aided biological activities such as anticancer, anxiolytic and analgesic activities using Schrödinger Maestro V11.1 software and PASS and ADME/T.
7. Laboratory Management Able to manage laboratory with standard operating procedure, keeping records, data management etc
8. Statistical packages SPSS, GraphPad prism.
9. Manuscript writing Capable of writing manuscript, and published in a journal.

1. Nasrin, Suaad, Mohammad Nazmul Islam, Mohammed Abu Tayab, Mst Samima Nasrin, Md Abu Bakar Siddique, Talha Bin Emran, and ASM Ali Reza. "Chemical profiles and pharmacological insights of Anisomeles indica Kuntze An experimental chemico-biological interaction." Biomedicine & Pharmacotherapy 149 (2022) 112842.

2. Uddin, ABM Neshar, Farhad Hossain, ASM Ali Reza, Mst Samima Nasrin, and AHM Khurshid Alam. "Traditional uses, pharmacological activities, and phytochemical constituents of the genus Syzygium A review." Food Science & Nutrition (2022).

3. A. S. M. Ali Reza, Mst. Samima Nasrin, Md. Amjad Hossen, Md. Atiar Rahman, Ibrahim Jantan, Md. Areeful Haque & Eduardo Sobarzo-Sánchez (2021) Mechanistic insight into immunomodulatory effects of food-functioned plant secondary metabolites, Critical Reviews in Food Science and Nutrition, DOI 10.1080/10408398.2021.2021138.

4. Islam, Md Shahidul, Md Mamunur Rashid, AM Abu Ahmed, A.S.M. Ali Reza, Md Atiar Rahman, and Tasrina Rabia Chowdhury. "The food ingredients of different extracts of Lasia spinosa (L.) Thwaites can turn it into a potential medicinal food." NFS Journal (2021).

5. Ahmed, AM Abu, Md Atiar Rahman, Md Amjad Hossen, ASM Ali Reza, Md Shahidul Islam, Md Mamunur Rashid, Md Khalid Juhani Rafi, Md Tanvir Ahmed Siddiqui, Abdullah Al-Noman, and Md Nazim Uddin. "Epiphytic Acampe ochracea orchid relieves paracetamol-induced hepatotoxicity by inhibiting oxidative stress and upregulating antioxidant genes in in vivo and virtual screening." Biomedicine & Pharmacotherapy 143 (2021) 112215.

6. Reza, ASM Ali, Nahid Sikdar, Mst Samima Nasrin, Md Atiar Rahman, Abu Montakim Tareq, AHM Khurshid Alam, Sanjida Sharmin, and Mohammed Abu Sayeed. "Knowledge, Attitude, Perception of Biological Science and Healthcare Professional Students to Complementary and Alternative Medicine Health Belief and Practice in Southeastern Region of Bangladesh A Comparative Study." Bangladesh Pharmaceutical Journal 24, no. 2 (2021) 159-167.

7. Rahman, Md Mahbubur, AS M. Ali Reza, Muhammad Ali Khan, Khaled Mahmud Sujon, Rokshana Sharmin, Mamunur Rashid, Md Golam Sadik et al. "Unfolding the Apoptotic Mechanism of Antioxidant Enriched-Leaves of Tabebuia pallida (Lindl.) Miers in EAC Cells and Mouse Model." Journal of Ethnopharmacology (2021) 114297.

8. Hossen MA, Ali Reza AS, Amin MB, Nasrin MS, Khan TA, Rajib MH, Tareq AM, Haque MA, Rahman MA, Haque MA. Bioactive metabolites of Blumea lacera attenuate anxiety and depression in rodents and computer‐aided model. Food Science & Nutrition. 2021.

9. Reza AA, Haque MA, Sarker J, Nasrin MS, Rahman MM, Tareq AM, Khan Z, Rashid M, Sadik MG, Tsukahara T, Alam AK. Antiproliferative and antioxidant potentials of bioactive edible vegetable fraction of Achyranthes ferruginea Roxb. in cancer cell line. Food Science & Nutrition. 2021.

10. Jahan I, Uddin ABMN, Reza ASMA, Uddin MG, Hossain MS, Nasrin MS, et al. (2021) Tendencies and attitudes towards dietary supplements use among undergraduate female students in Bangladesh. PLoS ONE 16(4) e0249897. doi10.1371/journal.pone.0249897

11. Rahman, M.M.; Uddin, M.J.; Reza, A.S.M.A.; Tareq, A.M.; Emran, T.B.; Simal-Gandara, J. Ethnomedicinal Value of Antidiabetic Plants in Bangladesh A Comprehensive Review. Plants 2021, 10, 729. https//doi.org/10.3390/ plants10040729

12. Moni JN, Adnan M, Tareq AM, Kabir M, Reza AS, Nasrin M, Chowdhury KH, Sayem SA, Rahman MA, Alam AH, Alam SB. Therapeutic Potentials of Syzygium Fruticosum Fruit (Seed) Reflect Into An Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways. Life. 2021 Feb;11(2)155.

13. Hossen, Md Amjad, ASM Ali Reza, AM Abu Ahmed, Md Kamrul Islam, Israt Jahan, Rahni Hossain, Mohammad Forhad Khan, Mohammad Rifat Alam Maruf, Md Areeful Haque, and Md Atiar Rahman. "Pretreatment of Blumea lacera leaves ameliorate acute ulcer and oxidative stress in ethanol-induced Long-Evan rat A combined experimental and chemico-biological interaction." Biomedicine & Pharmacotherapy= Biomedecine & Pharmacotherapie 135 (2021) 111211-111211. (Elsevier).

14. Chowdhury, K.H.; Chowdhury, M.R.; Mahmud, S.; Tareq, A.M.; Hanif, N.B.; Banu, N.; A.S.M. Ali Reza.; Emran, T.B.; Simal-Gandara, J. Drug Repurposing Approach against Novel Coronavirus Disease (COVID-19) through Virtual Screening Targeting SARS-CoV-2 Main Protease. Biology, MDPI, 2020, 10, 2.

15. Babar ZU, Jaswir I, Maifiah MH, Ismail S, Raus RA, Tareq AM, Ahfter F, Faraque A, Reza AA, Sayeed MA, Hossain MM. The Thrombolytic and Cytotoxic Effects of Nigella sativa (L.) Seeds The Prophetic Medicine. International Journal of Halal Research. 2020 Dec 18;2(2)70-7.

16. Haque, Md Anwarul, A.S.M. Ali Reza, Mst Samima Nasrin, and Md Atiar Rahman. "Pleurotus highking mushrooms potentiate antiproliferative and antimigratory activity against triple-negative breast cancer cells by suppressing Akt signaling." Integrative Cancer Therapies 19 (2020) 1534735420969809. (Publisher SAGE, Q1 journal, Impact Factor- 2.65)

17. Rahman J, Tareq AM, Hossain M, Sakib SA, Islam MN, A.S.M. Ali Reza, Uddin AB, Hoque M, Nasrin M, Emran TB, Capasso R. Biological eValuation, DFT Calculations and Molecular Docking Studies on the Antidepressant and Cytotoxicity Activities of Cycas pectinata Buch.-Ham. Compounds. Pharmaceuticals. 2020 Sep;13(9)232. (Publisher MDPI)

18. Islam MN, Tasnim H, Arshad L, Haque MA, Tareq SM, Kamal AM, Rahman MM, A.S.M. Ali Reza, Chowdhury KA, Tareq AM. Stem extract of Albizia richardiana exhibits potent antioxidant, cytotoxic, antimicrobial, anti-inflammatory and thrombolytic effects through in vitro approach. Clinical Phytoscience. 2020 Dec;6(1)1-9. (Publisher Spinger-Nature)

19. Hossain, Kazi Helal, Md Atiar Rahman, Muhammad Taher, Jitbanjong Tangpong, Dina Hajjar, Walla Alelwani, Arwa A. Makki, and ASM Ali Reza. "Hot Methanol Extract of Leea Macrophylla (Roxb.) manages chemical-induced inflammation in Rodent Model." Journal of King Saud University-Science (2020). (Publisher Elsevier, Q1 journal, Impact Factor- 3.819)

20. Bristy, T.A.; Barua, N.; Montakim Tareq, A.; Sakib, S.A.; Etu, S.T.; Chowdhury, K.H.; Jyoti, M.A.; Aziz, M.A.I.; A. S. M. Ali Reza.; Caiazzo, E.; Romano, B.; Tareq, S.M.; Emran, T.B.; Capasso, R. Deciphering the Pharmacological Properties of Methanol Extract of Psychotria calocarpa Leaves by In Vivo, In Vitro and In Silico Approaches. Pharmaceuticals 2020, 13, 183. (Journal Pharmaceuticals, Publisher MDPI, Impact Factor- 4.69)

21. A. S. M. Ali Reza, Mst Samima Nasrin. Curculigo recurvata W.T.Aiton exhibits anti-nociceptive and anti-diarrheal effects in Albino mice and an in silico model (Animal Model and Experimental Medicine, Publisher Wiley, DOI https//doi.org/10.1002/ame2.12119).

22. Akter S, Shah M, Tareq AM, Nasrin MS, Rahman MA, Babar ZM, Haque MA, Royhan MJ, Mamun MN, A. S. M. Ali Reza, Emran TB. Pharmacological effect of methanolic and hydro-alcoholic extract of Coconut endocarp. (Journal of Advanced Biotechnology and Experimental Therapeutics, JABET)

23. Tareq AM, A. S. M. Ali Reza , Mst Samima Nasrin. Chemical profiles, pharmacological properties, and in silico studies provide new insights on Cycas pectinata (Heliyon, Publisher CellPress, Q1 Journal) DOI https//doi.org/10.1016/j.heliyon.2020.e04061

24. Tareq AM, Sohel M, Main Uddin M, Mahmud H, Hoque M, A. S. M Ali Reza, Nasrin MS, Kader FB, Emran TB. Possible neuropharmacological effects of Apis cerana indica beehive in the Swiss Albino mice.(Journal of Advance Biotechnology and Therapeutics).

25. Adnan M, Chy M, Uddin N, Kamal AT, Chowdhury KA, Rahman M, A. S. M. Ali Reza, Moniruzzaman M, Rony SR, Nasrin M, Azad M. Intervention in Neuropsychiatric Disorders by Suppressing Inflammatory and Oxidative Stress Signal and Exploration of In Silico Studies for Potential Lead Compounds from Holigarna caustica (Dennst.) Oken leaves. Biomolecules. 2020 Apr;10(4)561. (Publisher MDPI, Impact Factor- 4.69)

26. Sharmin, Sanjida, Tahmina Sifat, Afrina Azad, A. S. M Ali Reza, Mohammad Kaisarul Islam, and Mohammed Abu Sayeed. "Preliminary Phyto-pharmacological Investigation of Duabanga Grandiflora (Roxb. ex DC.) Walpers." (Bangladesh Pharmaceutical Journal)

27. Z. M. Babar, I. Jaswir, A. M. Tareq, A. S. M Ali Reza, W. M. Azizi, M. Hafidz, F. Ahfter, M. Hasan, S. Farhad, M. M. Rokib Uddin, S. Ja. Ichwan, Q. U. Ahmed, M. Taher & I. Mawa (2019) In vivo anxiolytic and in vitro anti-inflammatory activities of water-soluble extract (WSE) of Nigella sativa (L.) seeds, Natural Product Research, DOI 10.1080/14786419.2019.1667348, (Impact Factor- 1.91). Publisher Taylor & Francis

28. A. S. M. Ali Reza, Mst. Samima Nasrin, A. H. M. Khurshid Alam (2018) Phytochemicals, Antioxidants, and Cholinesterase Inhibitory Profiles of Elatostema Papillosum Leaves An Alternative Approach for Management of Alzheimer's Disease. J Neurol Neuromed (2018) 3(5) 19-22.

29. A. S. M. Ali Reza, Md. Josim Uddin, Md. Abdullah-Al-Mamun, Mohammad S. H. Kabir, Mst. Samima Nasrin, Sharmin Akhter, Md. Saiful Islam Arman and Md. Atiar Rahman "Antinociceptive and Anxiolytic and Sedative Effects of Methanol Extract of Anisomeles indica An Experimental Assessment in Mice and Computer Aided Models." Frontiers in Pharmacology (2018) 246. (Impact Factor- 3.38).

30. A. S. M. Ali Reza, Mohammad Shahadat Hossain, Sharmin Akhter, Md. Rezanur Rahman, Mst. Samima Nasrin, Md. Josim Uddin, Golam Sadik and A. H. M. Khurshid Alam “In vitro antioxidant and cholinesterase inhibitory activities of Elatostema papillosum leaves and correlation with their phytochemical profiles a study relevant to the treatment of Alzheimer's disease” BMC Complementary and Alternative Medicine, 2018, 13, 142. (Impact Factor 2.94). Publisher Spinger-Nature

31. Mobarak H, Meah MS, Sikder N, Tareq M, Azad A, Khatun R, Nasrin MS, Raihan MO, A. S. M. Ali Reza. Investigation of preliminary phytochemicals, analgesic, anti-arthritic, thrombolytic and Cytotoxic Activities of Begonia Roxburghii (Miq.) DC. Leaves. Med One. 2018 Feb 25;3(1).

32. Hossain, Mohammad Shahadat, A. S. M. Ali Reza, Md Masudur Rahaman, Mst Samima Nasrin, Mohammed Rasib Uddin Rahat, Md Rabiul Islam, Md Josim Uddin, and Md Atiar Rahman. "Evaluation of morning glory (Jacquemontia tamnifolia (L.) Griseb) leaves for antioxidant, antinociceptive, anticoagulant and cytotoxic activities." Journal of basic and clinical physiology and pharmacology 29.3 (2018) 291-299. (Impact Factor- 0.759). Publisher W de Gruyter

33. A.S.M Ali Reza, Sm Faysal Bellah, Md. Nur Islam, Md. Rezaul Karim, Md. Masudur Rahaman, Mst. Samima Nasrin, Md. Atiar Rahman, A.S.M Ali Reza, 2016. Evaluation of the cytotoxic, analgesic, antidiarrheal and phytochemical properties of Hygrophila spinosa (T. Anders) whole plant. Journal of Basic and Clinical Physiology and Pharmacology, Impact Factor 0.789 ). Publisher W de Gruyter

34. Prawej Ansari, Md. Josim Uddin, Md. Masudur Rahman, Md. Abdullah-Al-Mamun, Md. Rabiul Islam, Md. Hazrat Ali, A.S.M. Ali Reza . Anti-inflammatory, anti-diarrheal, thrombolytic and cytotoxic activities of an ornamental medicinal plant Persicaria orientalis. Journal of Basic and Clinical Physiology and Pharmacology, 2017 Jan 1;28(1)51-8. Publisher W de Gruyter.

35. Islam MR, Uddin MN A.S.M. Ali Reza, Rana MN, Farhana K. In vivo evaluation of analgesic activity of methanolic extract of Laportea interrupea (L.) leaves. J Chem Pharm Res. 2014;6(1)552-6.

36. Md. Rabiul Islam, A.S.M. Ali Reza, Md. Sajjad Hossain and Mst. Kaniz Farhana. In vitro Evaluation of Cytotoxic and Thrombolytic Activities of Oroxylum indicum (Linn.). Bangladesh Pharmaceutical Journal 17(1) 70-74, 2014.

1. Evaluation of in vitro antioxidant, anti-inflamatory and cytotoxic activities of chloroform extract of Solanum atropurpureum and in silico ADME/T test and PASS prediction of bioactive compounds identified by GC-MS analysis. A. S. M. Ali Reza, Mst. Samima Nasrin, Md. Amjad Hossen, A. M. Abu Ahmed, Md. Shahidul Islam, Md Mamunur Rashid, Md. Atiar Rahman The Bangladesh Society for Biochemistry and Molecular Biology Conference 2019, 27-28 April 2019, Department of Biochemistry & Molecular Biology, University of Chittagong

Poster Presentation
1. Dietary supplement use among undergraduate female students in private and public universities located in Chittagong region Bangladesh. Israt Jahan, A.S. M. Ali Reza, Md. Amjad Hossen, Mst. Samima Nasrin Md. Atiar Rahman. The Bangladesh Society for Biochemistry and Molecular Biology Conference 2019, 27-28 April 2019, Department of Biochemistry & Molecular Biology, University of Chittagong
2. Development of potential BACE1 & acetylcholinesterase inhibitors for Alzheimer’s disease treatment of some isolated compounds from Borreria hispida (L) A.S.M Ali Reza, Sajal Chakraborty, Abu Montakim Tareq, Dipannita Chowdhury. The Bangladesh Society for Biochemistry and Molecular Biology Conference 2019, 27-28 April 2019, Department of Biochemistry & Molecular Biology, University of Chittagong.
3. Evaluation of in vivo anxiolytic, anti-depressant, sedative and in vitro anti-inflammatory activities of methanol extract and its different fractions of Lasia spinosa(L.)Thw. Mahfuz Ahmed Sakib, Mst. Samima Nasrin,Md. Atiar Rahman, A. S. M. Ali Reza. BSBMB Conference 2019, Department of Biochemistry & Molecular Biology, University of Chittagong.

Merit Scholarship Provost Awards for securing First Class in Bachelor of Pharmacy (Hon’s) examination in 2009 from Rajshahi University, Rajshahi-6205, Bangladesh. and (Gold Medal) at post graduate level in 2010.
Merit Scholarship in undergraduate level (2006) from Rajshahi University.